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Research Article

Biosafety and biocompatibility assessment of Prussian blue nanoparticles in vitro and in vivo

    Zhou Wang‡

    College of Biology, Hunan University, Changsha 410082, China

    ‡Authors contributed equally and should be regarded as co-first authors

    Search for more papers by this author

    ,
    Ying Long‡

    College of Biology, Hunan University, Changsha 410082, China

    ‡Authors contributed equally and should be regarded as co-first authors

    Search for more papers by this author

    ,
    Jialong Fan

    College of Biology, Hunan University, Changsha 410082, China

    ,
    Chang Xiao

    College of Biology, Hunan University, Changsha 410082, China

    ,
    Chunyi Tong

    College of Biology, Hunan University, Changsha 410082, China

    ,
    Chenglin Guo

    Key Laboratory of Study & Discovery of Small Targeted Molecules of Hunan Province School of Medicine, Hunan Normal University, Changsha 410125, PR China

    ,
    Xinyi Chen

    Key Laboratory of Study & Discovery of Small Targeted Molecules of Hunan Province School of Medicine, Hunan Normal University, Changsha 410125, PR China

    ,
    Bin Liu

    *Author for correspondence: Tel.: +86 731 89720939;

    E-mail Address: binliu2001@hotmail.com

    College of Biology, Hunan University, Changsha 410082, China

    NHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Department of Physiology & Pathophysiology, School of Basic Medical Sciences, Ningxia Medical University, 750004 Yinchuan, PR China

    &
    Xiaoping Yang

    **Author for correspondence: Tel.: +86 731 89720939;

    E-mail Address: xiaoping.yang@hunnu.edu.cn

    Key Laboratory of Study & Discovery of Small Targeted Molecules of Hunan Province School of Medicine, Hunan Normal University, Changsha 410125, PR China

    Published Online:https://doi.org/10.2217/nnm-2020-0191

    Aim: To investigate the effects of the different morphological characteristics of Prussian blue nanoparticles (PB NPs) on their biocompatibility and biosafety. Materials & methods: PB NPs with different sizes, shapes and charges were synthesized and their biosafety and biocompatibility performance were systematically compared in vitro and in vivo. Results: Increased size and positive charge of PB NPs adversely affected cell viability, while improving their peroxidase activity and photothermal conversion efficiency. In vivo analysis demonstrated good biocompatibility of PB NPs, without retention in the organs, but increased size retarded their metabolism. Meanwhile, increased size and positive charge adversely affected hepatic and renal function. Conclusion: This comprehensive exploration of biosafety and biocompatibility provides strong evidences for the use of PB NPs as nanodrug carrier and/or imaging agent.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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