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Research Article

Concentration-dependent protein adsorption at the nano–bio interfaces of polymeric nanoparticles and serum proteins

    Tian-Xu Zhang

    State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

    Authors contributed equally

    Search for more papers by this author

    ,
    Guan-Yin Zhu

    State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

    Authors contributed equally

    Search for more papers by this author

    ,
    Bo-Yao Lu

    State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

    ,
    Chao-Liang Zhang

    State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

    &
    Qiang Peng

    *Author for correspondence: Tel.: +86 28 85 501 484; Fax: +86 28 85 501 484;

    E-mail Address: qiangpengzz@scu.edu.cn

    State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

    Published Online:https://doi.org/10.2217/nnm-2017-0238

    Aim: A comprehensive understanding of nanoparticle (NP)-protein interaction (protein corona formation) is required. So far, many factors influencing this interaction have been investigated, like size and ζ potential. However, NPs exposure concentration has always been ignored. Herein, we aim to disclose the correlation of NPs exposure concentration with protein adsorption. Materials & methods: Four polymeric NPs systems possessing similar sizes (230 ± 20 nm) but varied ζ potentials (−30 ∼ +40 mv) were prepared. Physicochemical properties and protein adsorption upon NP–protein interaction were characterized. Results: Protein adsorption capacity and adsorbed protein types were NPs concentration-dependent. Conclusion: Considering the critical impacts of protein adsorption on NPs delivery, our work could be an urgent warning about the possible risks of dosage adjustment of nanoformulations.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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