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Research Article

Nrf2 protects against oxidative stress induced by SiO2 nanoparticles

    Wei Liu‡

    Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control & Prevention, Shenzhen 518055, China

    ‡Authors contributed equally

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    ,
    Tao Hu‡

    Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control & Prevention, Shenzhen 518055, China

    Medical School of Hunan Normal University, Changsha, 410013, China

    ‡Authors contributed equally

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    ,
    Li Zhou‡

    Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control & Prevention, Shenzhen 518055, China

    ‡Authors contributed equally

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    ,
    Desheng Wu

    Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control & Prevention, Shenzhen 518055, China

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    ,
    Xinfeng Huang

    Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control & Prevention, Shenzhen 518055, China

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    ,
    Xiaohu Ren

    Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control & Prevention, Shenzhen 518055, China

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    ,
    Yuan Lv

    Medical School of Hunan Normal University, Changsha, 410013, China

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    ,
    Wenxu Hong

    Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control & Prevention, Shenzhen 518055, China

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    ,
    Guanqin Huang

    Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control & Prevention, Shenzhen 518055, China

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    ,
    Zequn Lin

    Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control & Prevention, Shenzhen 518055, China

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    &
    Jianjun Liu

    *Author for correspondence: Tel.: +86 0755 2550 8584; Fax: +86 0755 2550 8584;

    E-mail Address: junii8@126.com

    Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control & Prevention, Shenzhen 518055, China

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    Published Online:19 Sep 2017https://doi.org/10.2217/nnm-2017-0046

    Abstract

    Aim: The aim of our study was to explore the role of nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2) on the exposure of SiO2 nanoparticles (NPs) and its influence. Materials & methods: To understand the mechanism of NP-induced oxidative stress, the involvement of oxidative-stress-responding transcription factors and the Nrf2/antioxidant reactive element (ARE) signaling pathway in the toxicity of SiO2 NPs’ exposure was investigated via in vivo and in vitro models. Results: A549 cells showed a significant cytotoxic effect while A549-shNrf2 cells showed decreased cell viability after nm-SiO2 exposure. SiO2 NPs’ exposure activated the Nrf2/ARE signaling pathway. Nrf2−/− exposed mice showed increased reactive oxygen species, 8-hydroxyl deoxyguanosine level and decreased total antioxidant capacity. Nrf2/ARE signaling pathway activation disrupted, leading inhibition of heme oxygenase-1 and upregulation of PKR-like endoplasmic-reticulum-regulated kinase. Conclusion: Our findings suggested that Nrf2 could protect against oxidative stress induced by SiO2 NPs, and the Nrf2/ARE pathway might be involved in mild-to-moderate SiO2 NP-induced oxidative stress that was evident from dampened activity of Nrf2.

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