Research Article

Upregulated genes at 2q24 gains as candidate oncogenes in hepatoblastomas

Tatiane Cristina Rodrigues

Department of Genetics & Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil

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Felipe Fidalgo

International Center for Research, A. C. Camargo Cancer Center, São Paulo, Brazil

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Cecilia Maria Lima da Costa

Department of Pediatric Oncology, A. C. Camargo Cancer Center, São Paulo, Brazil

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Elisa Napolitano Ferreira

International Center for Research, A. C. Camargo Cancer Center, São Paulo, Brazil

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Isabela Werneck da Cunha

Department of Pathology, A. C. Camargo Cancer Center, São Paulo, Brazil

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Dirce Maria Carraro

International Center for Research, A. C. Camargo Cancer Center, São Paulo, Brazil

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Ana Cristina Victorino Krepischi

Department of Genetics & Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil

International Center for Research, A. C. Camargo Cancer Center, São Paulo, Brazil

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Carla Rosenberg

*Author for correspondence:

E-mail Address: carlarosenberg@uol.com.br

Department of Genetics & Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil

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Published Online:19 Dec 2014https://doi.org/10.2217/fon.14.149

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Abstract

ABSTRACT

Aim: Cytogenetic data of hepatoblastomas, a rare embryonal tumor of the liver, mostly consist of descriptions of whole-chromosome aneuploidies and large chromosome alterations. High-resolution cytogenetics may provide clues to hepatoblastoma tumorigenesis and indicate markers with clinical significance. Patients & methods: We used array-CGH (180K) to screen for genomic imbalances in nine hepatoblastomas. Additionally, we investigated the expression pattern of selected genes exhibiting copy number changes. Results: Analysis showed mainly whole-chromosome or chromosome-arm aneuploidies, but some focal aberrations were also mapped. Expression analysis of 48 genes mapped at one 10 Mb amplification at 2q24 revealed upregulation of DAPL1, ERMN, GALNT5, SCN1A and SCN3A in the set of tumors compared with differentiated livers. Conclusion: These genes appear as candidates for hepatoblastoma tumorigenesis.

Keywords:

Papers of special note have been highlighted as: • of interest; •• of considerable interest

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Published online 19 December 2014

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Acknowledgements

The authors thank the Biobank from the A.C. Camargo Cancer Center (São Paulo, Brazil) for providing tumor samples, and the patients and their families for their precious collaboration.

Financial & competing interests disclosure

The present study was supported by FAPESP grants 2011/24007-9, 2009/00898-1, 2013/08028-1 and 2006/00054-0. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct

The authors state that they have obtained appropriate institutional review board approval and have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, informed consent has been obtained from the participants involved.

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