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Antimicrobial photodynamic activity of hypericin against methicillin-susceptible and resistant Staphylococcus aureus biofilms

    Isabel García

    *Author for correspondence:

    E-mail Address: igarcial@us.es

    Department of Microbiology, School of Medicine, Universidad de Sevilla, Sevilla, Spain

    Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015), Instituto de Salud Carlos III, Madrid, Spain

    ,
    Sofía Ballesta

    Department of Microbiology, School of Medicine, Universidad de Sevilla, Sevilla, Spain

    Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015), Instituto de Salud Carlos III, Madrid, Spain

    ,
    Yolanda Gilaberte

    Department of Dermatology, IIS Aragón, Hospital San Jorge, Huesca, Spain

    ,
    Antonio Rezusta

    Department of Microbiology, Hospital Universitario Miguel Servet, IIS Aragón, Universidad de Zaragoza, Zaragoza, Spain

    &
    Álvaro Pascual

    Department of Microbiology, School of Medicine, Universidad de Sevilla, Sevilla, Spain

    Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015), Instituto de Salud Carlos III, Madrid, Spain

    Infectious Diseases & Clinical Microbiology Unit, Hospital Universitario Virgen Macarena, Sevilla, Spain

    Published Online:https://doi.org/10.2217/fmb.14.114

    ABSTRACT 

    Aim: To evaluate the effectiveness of the photodynamic therapy using hypericin (HYP) against both planktonic and biofilm-forming Staphylococcus aureus. Materials & methods: HYP photoactivity was evaluated against methicillin-susceptible and resistant S. aureus. Bacterial suspension or biofilm were preincubated with HYP and subjected to LED illumination. Viable bacteria were determined by colony counting. Results: Preincubation with HYP (5 min) plus light exposure (10 min) showed bactericidal effect against planktonic methicillin-susceptible S. aureus and methicillin-resistant S. aureus. Longer preincubation times (24 h) and time light exposure (30 min) were required to reach HYP-photoactivity against S. aureus biofilms. HYP-photoactivity was correlated to the biofilm production. Conclusion: HYP could be a potential photosensitizer for the inactivation of staphylococcal biofilms forming on the surfaces accessible to visible light.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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