Antimicrobial photodynamic activity of hypericin against methicillin-susceptible and resistant Staphylococcus aureus biofilms
Abstract
ABSTRACT
Aim: To evaluate the effectiveness of the photodynamic therapy using hypericin (HYP) against both planktonic and biofilm-forming Staphylococcus aureus. Materials & methods: HYP photoactivity was evaluated against methicillin-susceptible and resistant S. aureus. Bacterial suspension or biofilm were preincubated with HYP and subjected to LED illumination. Viable bacteria were determined by colony counting. Results: Preincubation with HYP (5 min) plus light exposure (10 min) showed bactericidal effect against planktonic methicillin-susceptible S. aureus and methicillin-resistant S. aureus. Longer preincubation times (24 h) and time light exposure (30 min) were required to reach HYP-photoactivity against S. aureus biofilms. HYP-photoactivity was correlated to the biofilm production. Conclusion: HYP could be a potential photosensitizer for the inactivation of staphylococcal biofilms forming on the surfaces accessible to visible light.
Papers of special note have been highlighted as: • of interest; •• of considerable interest
References
- 1 . Staphylococcus aureus biofilms: properties, regulation, and roles in human. Virulence 2(5), 445–459 (2011).
- 2 . Antibiotic resistance of bacteria in biofilms. Lancet 358(9276), 135–138 (2001).
- 3 . Biofilms: survival mechanisms of clinically relevant microorganisms. Clin. Microbiol. Rev. 15(2), 167–193 (2002).
- 4 . New trends in diagnosis and control strategies for implant infections. Int. J. Artif. Organs 34(9), 727–736 (2011).• This review discusses the use of new methods for the diagnosis of biofilm infections and new strategies to prevent or control infections related to biofilms.
- 5 Protease-stable polycationic photosensitizer conjugates between polyethyleneimine and chlorin (e6) for broad-spectrum antimicrobial photoinactivation. Antimicrob. Agents. Chemother. 50(4), 1402–1410 (2006).
- 6 . In vitro photodynamic eradication of Pseudomonas aeruginosa in planktonic and biofilm culture. Photochem. Photobiol. 85(1), 137–143 (2009).
- 7 Effects of 5-aminolevulinic acid-mediated photodynamic therapy on antibiotic-resistant staphylococcal biofilm: an in vitro study. J. Surg. Res. 184(2), 1013–1021 (2013).
- 8 . Multiresistant strains are as susceptible to photodynamic inactivation as their naïve counterparts: protoporphyrin IX-mediated photoinactivation reveals differences between methicillin-resistant and methicillin-sensitive Staphylococcus aureus strains. Photomed. Laser Surg. 32(3), 121–129 (2014).•• Article that evaluates the antimicrobial photodynamic therapy against a significant number of methicillin-susceptible and resistant S. aureus clinical isolates.
- 9 Photodynamic inactivation of biofilm: taking a lightly colored approach to stubborn infection. Expert Rev. Anti Infect. Ther. 11(7), 669–693 (2013).•• This review focuses on the aspects of antimicrobial photodynamic therapy that are designed to increase its efficiency against biofilms.
- 10 . Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms. J. Antimicrob. Chemother. 57(4), 680–684 (2006).
- 11 . Antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection. Lasers Surg. Med. 39, 59–66 (2007).
- 12 . Toluidine blue-mediated photodynamic effects on staphylococcal biofilms. Antimicrob. Agents Chemother. 52(1), 299–305 (2008).• This study examines the photodynamic therapy effects on the viability and architecture of staphylococcal biofilms.
- 13 . The effect of photodynamic treatment combined with antibiotic action or host defence mechanisms on Staphylococcus aureus biofilms. Biomaterials 30(18), 3158–3166 (2009).
- 14 Photodynamic action of Tri-meso (N-methyl-pyridyl), meso (N-tetradecyl-pyridyl) porphine on Staphylococcus epidermidis biofilms grown on Ti6Al4V alloy. Int. J. Artif. Organs. 33(9), 636–645 (2010).
- 15 Non-aggregated Ga (III)-phthalocyanines in the photodynamic inactivation of planktonic and biofilm cultures of pathogenic microorganisms. Photochem. Photobiol. Sci. 10(1), 91–102 (2011).
- 16 . Susceptibility of Candida albicans, Staphylococcus aureus and Streptococcus mutans biofilms to photodynamic inactivation: an in vitro study. Lasers Med Sci. 26(3), 341–348 (2011).
- 17 . Targeted photodynamic therapy – a promising strategy of tumor treatment. Photochem. Photobiol. Sci. 10(7), 1097–1109 (2011).
- 18 . A Phase II placebo-controlled study of photodynamic therapy with topical hypericin and visible light irradiation in the treatment of cutaneous T-cell lymphoma and psoriasis. J. Am. Acad. Dermatol. 63(6), 984–990 (2010).
- 19 . Hypericin-mediated photodynamic antimicrobial effect on clinically isolated pathogens. Photochem. Photobiol. 88(3), 626–632 (2012).•• This study evaluates the photodynamic antimicrobial effect of hypericin against both Gram-positive and Gram-negative bacteria.
- 20 On the mechanism of Candida spp. photoinactivation by hypericin. Photochem. Photobiol. Sci. 11(6), 1099–1107 (2012).
- 21 In vitro fungicidal photodynamic effect of hypericin on Candida species. Photochem. Photobiol. 88(3), 613–619 (2012).
- 22 . Photodynamic effect of hypericin on the microorganisms and primary human fibroblasts. Photodiagnosis Photodyn. Ther. 10(2), 150–155 (2013).
- 23 . Antibacterial photodynamic therapy using water-soluble formulations of hypericin or mTHPC is effective in inactivation of Staphylococcus aureus. Photochem. Photobiol. Sci. 9(3), 365–369 (2010).
- 24 Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J. Clin. Microbiol. 22(6), 996–1006 (1985).
- 25 . Modified microtiter-plate test for quantification of staphylococcal biofilm formation. J. Microbial Methods 40(2), 175–179 (2000).
- 26 . Carriage of both the fnbA and fnbB genes and growth at 37°C promote FnBP-mediated biofilm development in methicillin-resistant Staphylococcus aureus clinical isolates. J. Med. Microbiol. 58(Pt 4), 399–402 (2009).
- 27 Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing, 23 informational supplement. CLSI M100-S23. Clinical and Laboratory Standards Institute, PA, USA (2013).
- 28 . Bactericidal effect of photodynamic inactivation against methicillin-resistant and methicillin-susceptible Staphylococcus aureus is strain-dependent. J. Photochem. Photobiol. B. 90(1), 57–63 (2008).
- 29 Effect of different pre-irradiation times on curcumin-mediated photodynamic therapy against planktonic cultures and biofilms of Candida spp. Arch. Oral Biol. 58, 200–210 (2013).
- 30 . Photodynamic inactivation for controlling Candida albicans infections. Fungal Biol. 116(1), 1–10 (2012).
- 31 . Photosensitization of in vitro biofilms by toluidine blue O combined with light-emitting diode. Eur. J. Oral Sci. 114(1), 64–69 (2006).
- 32 Association between methicillin susceptibility and biofilm regulation in Staphylococcus aureus isolates from device-related infections. J. Clin. Microbiol. 45(5), 1379–1388 (2007).• This study investigates the contribution of genetic background to biofilm development and the relationship between methicillin susceptibility and biofilm phenotype in clinical isolates of S. aureus.
- 33 . A spectrum of changes occurs in peptidoglycan composition of glycopeptide-intermediate clinical Staphylococcus aureus isolates. Antimicrob. Agents Chemother. 45(1), 280–287 (2001).
- 34 . Effects of growth phase and extracellular slime on photodynamic inactivation of Gram-positive pathogenic bacteria. Antimicrob. Agents Chemother. 48(6), 2173–2178 (2004).
- 35 . Efflux pump inhibitor potentiates antimicrobial photodynamic inactivation of Enterococcus faecalis biofilm. Photochem. Photobiol. 88(6), 1343–1349 (2010).