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Lactobacillus acidophilus supernatant alleviates osteoporosis by upregulating colonic SERT expression

    Jianhua Zhai

    Department of Emergency, Tianjin Medical University General Hospital, Tianjin, China.

    ,
    Siyuan Sun

    Department of Gastroenterology & Hepatology, Tianjin Medical University General Hospital, Tianjin, China

    ,
    Jing Cheng

    Department of Orthointernal, Tianjin Hospital, Tianjin, China

    ,
    Jing Wang

    Department of Gastroenterology & Hepatology, Tianjin Medical University General Hospital, Tianjin, China

    ,
    Ge Jin

    Department of Gastroenterology & Hepatology, Tianjin Medical University General Hospital, Tianjin, China

    ,
    Xiuxiu Xu

    Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, China

    ,
    Xiaotong Liu

    Department of Gastroenterology & Hepatology, Tianjin Medical University General Hospital, Tianjin, China

    ,
    Jingwen Zhao

    Department of Gastroenterology & Hepatology, Tianjin Medical University General Hospital, Tianjin, China

    ,
    Chen Chen

    Department of Geriatric Medicine, Tianjin Medical University General Hospital, Tianjin, China

    ,
    Weilong Zhong

    *Author for correspondence:

    E-mail Address: zhongweilong@tmu.edu.cn

    Department of Gastroenterology & Hepatology, Tianjin Medical University General Hospital, Tianjin, China

    &
    Bangmao Wang

    **Author for correspondence:

    E-mail Address: mwang02@tmu.edu.cn

    Department of Gastroenterology & Hepatology, Tianjin Medical University General Hospital, Tianjin, China

    Published Online:https://doi.org/10.2217/fmb-2022-0211

    Aims: To investigate the involvement of serotonin transporter (SERT) in colonic epithelial cells in the anti-osteoporosis role of Lactobacillus acidophilus (LA) supernatant (LAS). Methods: The abundance of fecal LA and bone mineral density (BMD) in patients with osteoporosis (OP) or severe osteoporosis were assessed. The protective role of LA in osteoporosis and the expression of SERT and relative signaling were evaluated. Results: Abundance of fecal LA was decreased in patients with severe OP and was positively correlated with BMD. Supplementing LAS to mice alleviated senile osteoporosis. In vitro, NOD2/RIP2/NF-κB signaling was inhibited by LAS due to increased SERT expression. Conclusion: LAS alleviates OP in mice by producing protective metabolites and upregulating SERT expression and represents a promising therapeutic agent.

    Tweetable abstract

    The involvement of SERT in the antiosteoporosis role of LAS remains unexplored. This study indicates that LAS alleviates OP by upregulating colonic SERT expression via NOD2/RIP2/NF-κB signaling.

    Papers of special note have been highlighted as: • of interest

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