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Incidence of virulence determinants and antibiotic resistance in lactic acid bacteria isolated from food products

    Mike T Keter

    Department of Microbiology & Biotechnology, Kenyatta University, 43844-00100, Kenya

    ,
    Nancy M El Halfawy

    *Author for correspondence: Tel.: +20 102 041 6146;

    E-mail Address: nancy.elhalfawy@alexu.edu.eg

    Department of Botany & Microbiology, Faculty of Science, Alexandria University, 21568, Egypt

    &
    Moustafa Y El-Naggar

    Department of Botany & Microbiology, Faculty of Science, Alexandria University, 21568, Egypt

    Published Online:https://doi.org/10.2217/fmb-2021-0053

    Background: Lactic acid bacteria (LAB) confer beneficial health effects in humans. However, the safety of these bacteria and their potential to spread resistance in the environment must be evaluated. Materials & methods: Fifty-three LAB were isolated from different food samples and assessed for the prevalence of virulence determinants and antibiotic resistance profile. Results: Multiple resistance was reported for Lactobacillus brevis MIM04, having revealed phenotypic resistance to vancomycin (MIC >128 μg/ml), ampicillin, cefotaxime, oxacillin and gentamicin. Virulence traits (cylA, gelE, esp and agg) were detected using specific primers. Enterococcus faecium CHE32, Lactobacillus plantarum CHE37 and E. faecium MLK68 lack virulence genes, possess antimicrobial activity and survive in low pH and bile salt conditions. Conclusion: Isolated LAB revealed probiotic properties.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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