Preliminary Communication

Identification and regulation pattern analysis of long noncoding RNAs in meibomian gland carcinoma

Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, PR China

^‡Authors contributed equally

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Jiayan Fan^‡

Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, PR China

^‡Authors contributed equally

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Renbing Jia

Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, PR China

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Yixiong Zhou

Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, PR China

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Shengfang Ge

Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, PR China

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Ge Zhang

Department of Neurology, Luoyang Dong Fang Hospital, The Third Affiliated Hospital of Henan University of Science & Technology, Luoyang, Henan 471003, PR China

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Haibo Wang

*Author for correspondence:

E-mail Address: whbde@163.com

Institute of Cardiovascular Disease, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China

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Xianqun Fan

**Author for correspondence:

E-mail Address: fanxq@sh163.net

Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, PR China

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Published Online:7 Dec 2018https://doi.org/10.2217/epi-2018-0182

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Abstract

Aim: To identify long noncoding RNAs (lncRNAs) and to elucidate regulation patterns of lncRNAs in meibomian gland carcinoma (MGC). Materials & methods: We used RNA-Seq, gene ontology, ClueGO, Ingenuity Pathway Analysis and co-expression network analyses to profile the expression and regulation patterns of lncRNAs and mRNAs in MGC. Results: We identified 500 lncRNAs and 326 mRNAs as differentially expressed. Co-expression regulatory networks with lncRNAs and mRNAs were constructed. The differentially expressed mRNAs and lncRNAs were enriched by fundamental biological functions that are implicated in the inflammatory signaling pathway and tumor proliferation (IL6 and PTGS2). Conclusion: LncRNAs might play important roles via the competing endogenous RNA regulation pattern in MGC tumorigenesis and contribute to the molecular pathogenesis of MGC.

Keywords:

Papers of special note have been highlighted as: • of interest; •• of considerable interest

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Vol. 11, No. 4

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History

Received 25 October 2018

Accepted 26 November 2018

Published online 7 December 2018

Published in print March 2019

Information

Keywords

Author contributions

Conceptualization was done by H Wang and X Fan. Formal analysis was done by X Song and J Fan. Funding acquisition was done by X Song, G Zhang, J Fan, R Jia and H Wang. Project administration was done by Y Zhou, S Ge and H Wang. Visualization was done by J Fan, R Jia, Y Zhou and G Zhang. Original draft was written by X Song, R Jia, S Ge and H Wang. Review & editing was done by X Fan.

Financial & competing interests disclosure

This study was supported by grants from the National Natural Science Foundation of China (31200632 to X Song, 81300207 to H Wang, 81502481 and 81872339 to J Fan, 81570884 and 81770961 to R Jia), the Bureau of Luoyang City Science and Technology 2017 Medical and Health Program (1725001A-8 to G Zhang), Shanghai Ninth People's Hospital training programs (jyyq09201713 to J Fan) and young doctors’ innovation team (QC201805 to J Fan).

No writing assistance was utilized in the production of this manuscript.

Ethical disclosure

The clinical research protocol was approved by the local Ethics Committee and was in accordance with the principles of the Declaration of Helsinki. All samples were collected with patients’ informed consent, and there were no security or privacy violations in our study.

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