Research Article

hsa-mir-499 rs3746444 gene polymorphism is associated with susceptibility to breast cancer in an Iranian population

Mohsen Omrani

Cellular & Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

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Mohammad Hashemi

* Author for correspondence

Cellular & Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.

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Email the corresponding author at mhd.hashemi@gmail.com

Ebrahim Eskandari-Nasab

Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

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Seyed-Shahaboddin Hasani

Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

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Mohammad Ali Mashhadi

Department of Internal Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

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Farshid Arbabi

Department of Internal Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

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Mohsen Taheri

Genetics of Non Communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

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Published Online:12 Feb 2014https://doi.org/10.2217/bmm.13.118

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Abstract

Aim: Our study aimed to evaluate the possible association between four miRNA polymorphisms, hsa-miR-146a (rs2910164 G>C), hsa-miR-499 (rs3746444 T>C) and hsa-miRNA-196a2 (rs11614913 C>T and rs185070757 T>G), and susceptibility to breast cancer in an Iranian population. Materials & methods: In this case–control study we enrolled 236 patients with breast cancer and 203 healthy individuals. Tetra primer amplification refractory mutation system PCR was applied for genotyping the four miRNA SNPs. Results: Our study indicated that the hsa-mir-499 rs3746444 CC homozygote increased the risk of breast cancer in the dominant (odds ratio [OR]: 2.42; 95% CI: 1.43–4.09; p = 0.001; CC vs TT) and recessive (OR: 2.48; 95% CI: 1.49–4.13; p = 0.004; CC vs TT+TC) inheritance models tested. In addition, the rs3746444 C allele increased the risk of breast cancer (OR: 1.71; 95% CI: 1.27–2.29; p = 0.0004) in comparison with the T allele. However, distribution of the rs2910164 G>C, rs11614913 C>T and rs185070757 T>G genotypes was not statistically different between cases and controls (p > 0.05). Conclusion: Our findings demonstrated that the hsa-mir-499 rs3746444 polymorphism is associated with higher risk of developing breast cancer in our population.

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Papers of special note have been highlighted as: ▪ of interest ▪▪ of considerable interest

References

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Published online 12 February 2014

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Acknowledgements

This paper was based on the MSc thesis of M Omrani.

Financial & competing interests disclosure

The Deputy for Research, Zahedan University of Medical Sciences, provided funding for this research. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

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