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Regenerative Medicine

Using poly(lactide-co-glycolide) electrospun scaffolds to deliver cultured epithelial cells to the cornea

    Pallavi Deshpande

    Department of Engineering Materials, Kroto Research Institute, University of Sheffield, North Campus, Broad Lane, Sheffield, S3 7HQ, UK

    ,
    Rob McKean

    Department of Chemistry, University of Sheffield, Sheffield S3 7HF, UK

    ,
    Keith A Blackwood

    Department of Engineering Materials, Kroto Research Institute, University of Sheffield, North Campus, Broad Lane, Sheffield, S3 7HQ, UK

    ,
    Richard A Senior

    Department of Engineering Materials, Kroto Research Institute, University of Sheffield, North Campus, Broad Lane, Sheffield, S3 7HQ, UK

    ,
    Adekemi Ogunbanjo

    Department of Engineering Materials, Kroto Research Institute, University of Sheffield, North Campus, Broad Lane, Sheffield, S3 7HQ, UK

    ,
    Anthony J Ryan

    Department of Chemistry, University of Sheffield, Sheffield S3 7HF, UK

    &
    Published Online:https://doi.org/10.2217/rme.10.16

    Aims: To assess the potential of electrospun poly(lactide-co-glycolide) membranes to provide a biodegradable cell carrier system for limbal epithelial cells. Material & methods: 50:50 poly(lactide-co-glycolide) scaffolds were spun, sterilized and seeded with primary rabbit limbal epithelial cells. Cells were cultured on the scaffolds for 2 weeks and then examined by confocal microscopy, cryosectioning and scanning-electron microscopy. The tensile strength of scaffolds before and after annealing and sterilization was also studied. Results: The limbal cells had formed a continuous multilayer of cells on either side of the scaffold. Scaffolds with cells showed signs of the onset of degradation within 2 weeks in culture media at 37°C. Scaffolds that were annealed resulted in a more brittle and stiff mat. Conclusions: We suggest this carrier membrane could be used as a replacement for the human amniotic membrane in the treatment of limbal stem cell deficiency, lowering the risk of disease transmission to the patient.

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