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Research Articlecc iconby iconnc iconnd icon

Nanoparticle conversion to biofilms: in vitro demonstration using serum-derived mineralo-organic nanoparticles

    Tsui-Yin Wong‡

    Laboratory of Nanomaterials, Chang Gung University, Gueishan, Taoyuan 33302, Taiwan

    Center for Molecular & Clinical Immunology, Chang Gung University, Gueishan, Taoyuan 33302, Taiwan

    ‡Authors contributed equally

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    ,
    Hsin-Hsin Peng‡

    Laboratory of Nanomaterials, Chang Gung University, Gueishan, Taoyuan 33302, Taiwan

    Center for Molecular & Clinical Immunology, Chang Gung University, Gueishan, Taoyuan 33302, Taiwan

    ‡Authors contributed equally

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    ,
    Cheng-Yeu Wu

    Laboratory of Nanomaterials, Chang Gung University, Gueishan, Taoyuan 33302, Taiwan

    Center for Molecular & Clinical Immunology, Chang Gung University, Gueishan, Taoyuan 33302, Taiwan

    Research Center of Bacterial Pathogenesis, Chang Gung University, Gueishan, Taoyuan 33302, Taiwan

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    ,
    Jan Martel

    Laboratory of Nanomaterials, Chang Gung University, Gueishan, Taoyuan 33302, Taiwan

    Center for Molecular & Clinical Immunology, Chang Gung University, Gueishan, Taoyuan 33302, Taiwan

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    ,
    David M Ojcius

    Center for Molecular & Clinical Immunology, Chang Gung University, Gueishan, Taoyuan 33302, Taiwan

    Department of Biomedical Sciences, University of the Pacific, Arthur Dugoni School of Dentistry, San Francisco, CA 94103, USA

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    ,
    Fu-Yung Hsu

    Department of Materials Engineering, Ming Chi University of Technology, Taishan, New Taipei City 24301, Taiwan

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    &
    John D Young

    *Author for correspondence:

    E-mail Address: dingeyoung@hotmail.com

    Laboratory of Nanomaterials, Chang Gung University, Gueishan, Taoyuan 33302, Taiwan

    Center for Molecular & Clinical Immunology, Chang Gung University, Gueishan, Taoyuan 33302, Taiwan

    Laboratory of Cellular Physiology & Immunology, Rockefeller University, New York, NY 10021, USA

    Biochemical Engineering Research Center, Ming Chi University of Technology, Taishan, New Taipei City 24301, Taiwan

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    Published Online:2 Oct 2015https://doi.org/10.2217/nnm.15.171

    Abstract

    Aims: Mineralo-organic nanoparticles (NPs) detected in biological fluids have been described as precursors of physiological and pathological calcifications in the body. Our main objective was to examine the early stages of mineral NP formation in body fluids. Materials & methods: A nanomaterial approach based on atomic force microscopy, dynamic light scattering, electron microscopy and spectroscopy was used. Results: The mineral particles, which contain the serum proteins albumin and fetuin-A, initially precipitate in the form of round amorphous NPs that gradually grow in size, aggregate and coalesce to form crystalline mineral films similar to the structures observed in calcified human arteries. Conclusion: Our study reveals the early stages of particle formation and provides a platform to analyze the role(s) of mineralo-organic NPs in human tissues.

    Papers of special note have been highlighted as: •• of considerable interest

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