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Research Article

Complex encounters: nanoparticles in whole blood and their uptake into different types of white blood cells

    Daniela Baumann

    Max-Planck-Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany

    ,
    Daniel Hofmann

    Max-Planck-Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany

    ,
    Sven Nullmeier

    Institute of Anatomy, University of Magdeburg, Haus 43, Leipziger Straße 44, 39120 Magdeburg, Germany

    ,
    Patricia Panther

    Institute of Anatomy, University of Magdeburg, Haus 43, Leipziger Straße 44, 39120 Magdeburg, Germany

    ,
    Claudia Dietze

    Max-Planck-Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany

    ,
    Anna Musyanovych

    Max-Planck-Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany

    ,
    Sandra Ritz

    Max-Planck-Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany

    ,
    Katharina Landfester

    Max-Planck-Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany

    &
    Volker Mailänder

    * Author for correspondence

    Max-Planck-Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany. .

    Third Department of Medicine (Hematology, Oncology & Pneumology), University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstraße 1, 55131 Mainz, Germany

    Published Online:https://doi.org/10.2217/nnm.12.111

    Aim: A whole blood assay for evaluating the uptake of nanoparticles into white blood cells in order to close the gap between basic studies in cell culture and pharmacokinetic studies in animals was developed. Materials & methods: After drawing peripheral blood into standard blood collection vials with different anticoagulants, amino- and carboxy-functionalized polymeric styrene nanoparticles were added and uptake was evaluated by flow cytometry. Results: By counterstaining surface markers of leukocytes (e.g., monocytes, neutrophil granulocytes, B or T lymphocytes), investigations of different cell types can be conducted in a single run by flow cytometry. The authors demonstrated that anticoagulation should be done with heparin, and not EDTA, in order to prevent hampering of uptake mechanisms. Conclusion: By using heparinized whole blood, the authors demonstrated differences and usefulness of this assay for screening cellular uptake as it should occur in the bloodstream. Nevertheless, animal studies are warranted for final assessment of the nanoparticles.

    Original submitted 11 November 2011; Revised submitted 1 July 2012; Published online 31 August 2012

    Papers of special note have been highlighted as: ▪ of interest

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