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Function of glutaredoxin 3 (Grx3) in oxidative stress response caused by iron homeostasis disorder in Candida albicans

    Dan Zhang

    Key Laboratory of Molecular Microbiology & Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China

    ,
    Yijie Dong

    Key Laboratory of Molecular Microbiology & Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China

    The State Key Laboratory for Biology of Plant Disease & Insect Pests, Institute of Plant protection, Chinese Academy of Agricultural sciences, Beijing 100871, China

    ,
    Qilin Yu

    Key Laboratory of Molecular Microbiology & Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China

    ,
    Zhang Kai

    Key Laboratory of Molecular Microbiology & Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China

    ,
    Meng Zhang

    Key Laboratory of Molecular Microbiology & Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China

    ,
    Chang Jia

    Key Laboratory of Molecular Microbiology & Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China

    ,
    Chenpeng Xiao

    Key Laboratory of Molecular Microbiology & Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China

    ,
    Bing Zhang

    Key Laboratory of Molecular Microbiology & Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China

    ,
    Biao Zhang

    College of language and culture, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China

    &
    Mingchun Li

    *Author for correspondence:

    E-mail Address: nklimingchun@163.com

    Key Laboratory of Molecular Microbiology & Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China

    Published Online:https://doi.org/10.2217/fmb-2017-0098

    Aim: Glutaredoxin is a conserved oxidoreductase in eukaryotes and prokaryotes. This study aimed to determine the role of Grx3 in cell survival, iron homeostasis and the oxidative stress response in Candida albicans. Materials & methods: A grx3Δ/Δ mutant was obtained using PCR-mediated homologs recombination. The function of Grx3 was investigated by a series of biochemical methods. Results: Deletion of GRX3 impaired growth and cell cycle, disturbance of iron homeostasis and activated the oxidative stress response. Furthermore, disruption of GRX3 caused oxidative damage and growth defects of C. albicans. Conclusion: Our findings provide new insights into the role of GRX3 in C. albicans.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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